|
|
| Regel 11: |
Regel 11: |
| | |sample_volume = 250 µL (0.25 mL) plasma | | |sample_volume = 250 µL (0.25 mL) plasma |
| | |method = RIA | | |method = RIA |
| − | |freq = 1x/week | + | |freq = 1x/3w |
| | |delay = 1 dag | | |delay = 1 dag |
| | |unit = ng/L | | |unit = ng/L |
| Regel 35: |
Regel 35: |
| | {{Reflist}} | | {{Reflist}} |
| | | | |
| − | COMPARATIVE SEQUENCES OF THE CANINE AND FELINE ARGININE-VASOPRESSIN PROHORMONES. A.P. Santos1, A.W. Biondo2, D.D. Sisson3, S.TA. Lopes1, C. Souza1, P.F. Solter4. Departments of Small Animal Clinics of 1Universidade Federal de Santa Maria and 2Universidade Federal do Parana, Brazil; and Departments of 3Veterinary Clinical Medicine and 4Veterinary Pathobiology, University of Illinois, Urbana, IL, USA.
| + | ==Links== |
| − | | + | [[Wikipedia:Vasopressin]] |
| − | Vasopressin plays a key role in cardiovascular homeostasis, and is synthesized as a prohormone containing 4 peptide domains: a signal sequence, vasopressin nonapeptide, neurophysin II, and a C-terminal glycoprotein. Our laboratory is investigating the hormonal effects of heart failure in dogs and cats and has been attempting to verify, when it is not known, the nucleotide and amino acid sequences of relevant peptide hormones as tools for research. For that reason we sought to determine the structure of canine and feline vasopressin prohormone. Initially, PCR amplification of cDNA from feline and canine hypothalamus was done using consensus primers designed to regions upstream and downstream from the vasopressin sequence. This resulted in a 222 bp feline and canine cDNA product. Analysis of these sequences revealed that the nucleotides of feline and canine vasopressin nonapeptide differ slightly, but the amino acid sequence (CYFQNCPRG) is identical to that of most other mammalian species, except swine. Reconstruction of the complete canine prohormone from the newly available canine whole genome shotgun database at NCBI was subsequently done. Sequence homology of other canine and feline prohormone domains was not as well conserved as that of vasopressin nonapeptide. The results of this study corroborate the use of vasopressin immunoassays developed for other species with canine and feline vasopressin nonapeptide. These sequence data will also allow the design of specific vasopressin nucleotide probes for molecular studies and will be useful in the design of antibody probes to other vasopressin prohomone domains. | + | |
| − | | + | |
| | | | |
| | [[Category:LabWijzer]] | | [[Category:LabWijzer]] |
Versie van 5 okt 2012 om 16:23
| Benamingen en codes
|
| Omschrijving
|
ADH
|
| Synoniemen
|
AVP, Arginine vasopressine, Vasopressine, Argipressine
|
| Loinc
|
3126-0
|
| Code
|
DADH
|
| Aanvraagcode
|
2520
|
| Afname en methode
|
| Staal
|
EDTA-plasma ingevrozen
|
| Min volume (?)
|
250 µL (0.25 mL) plasma
|
| Methode
|
RIA
|
| Rapportering
|
| Rubriek
|
Research
|
| Subrubriek
|
Hormonen
|
| Frequentie
|
1x/3w
|
| Doorlooptijd
|
1 dag
|
| Eenheid
|
ng/L
|
| Alt. Eenheid
|
pg/mL
|
| Conversie
|
ng/L = pg/mL
|
|
Indicaties
Voor onderzoeksdoeleinden. Diagnostisch heeft de bepaling van ADH geen toepassing.
Afname
Fysiologie[1]
ADH wordt geproduceerd in de supra-optische en paraventriculaire kernen van de hypothalamus en gestockeerd in de hypofyseachterkwab.
De release ervan wordt in de eerste plaats gestimuleerd door hyperosmolaliteit van het plasma wat op zijn beurt in relatie staat tot de natrium plasmaconcentratie. ADH wordt ook vrijgezet wanneer het circulerende volume afneemt, hoewel in de eerste plaats volumewijzigingen gecontroleerd worden door het renine-angiotensinesysteem. Verminderde arteriële bloeddruk, verhoogde lichaamstemperatuur, pijn en sommige geneesmiddelen kunnen eveneens de ADHrelease bevorderen.
In de aanwezigheid van ADH wordt het distale deel van de distale tubulus contortus en de verzamelbuisjes permeabel voor water door de activatie van adenylcyclase. De hypertonociteit van het medullaire interstitium zorgt vervolgens voor de osmotische aandrijving van de waterresorptie.
Het is tevens een arteriële vasoconstrictor waardoor de bloeddruk verhoogt.
Referentie
- ↑ Fluid, Electrolyte, and Acid-Base Balance in Clinical Biochemistry of Domestic Animals; 6th Ed, 2008; Jiro Jerry Kaneko, John W. Harvey, Michael L. Bruss; ISBN 9780123704917
Links
Wikipedia:Vasopressin
